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AAT Bioquest

What is CRISPR-Cas9 technology?

Posted June 22, 2020


Answer

CRISPR-Cas9 is a genetic editing technique in molecular biology to modify the genomes of living organisms, which is adapted from a naturally occurring genome editing system in bacteria. By delivering a Cas9-gRNA complex into a cell, the cell’s genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added in vivo. This technology has found extensive applications in various areas, such as biomedicine, disease diagnosis and treatment, gene knockdown/activation, RNA editing etc.

Additional resources

Helixyte™ Green *10,000X Aqueous PCR Solution*

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

Doudna, J. A., & Charpentier, E. (2014). The new frontier of genome engineering with CRISPR-Cas9. Science, 346(6213), 1258096.

Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281.