AAT Bioquest

What is DNA fragmentation?

Posted February 16, 2022


DNA fragmentation refers to the process of separating or breaking DNA strands into pieces. This can occur spontaneously or it can be done intentionally in a laboratory or by cells themselves.

Spontaneous DNA fragmentation is a natural fragmentation process that occurs during apoptosis or programmed cell death. Also known as apoptotic DNA fragmentation, this spontaneous process is a biochemical hallmark of apoptosis.

Intentional DNA fragmentation in the laboratory is often performed before library construction or subcloning for DNA sequences. DNA is fragmented in the laboratory using a variety of methods such as needle sheer, passage through a pressure cell, point-sink shearing, sonication, or nebulization.

Additional resources

Types, Causes, Detection and Repair of DNA Fragmentation in Animal and Human Sperm Cells

Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Green Fluorescence*