The main goal of the polymerase chain reaction (PCR) is to rapidly amplify a specific sample of DNA/RNA from a single copy to multiple copies in vitro. Amplification is achieved by a series of four steps:
After completion of the first cycle, the process is repeated generally between 25 and 35 times, resulting in the exponential amplification of the original sample of DNA. To successfully amplify a segment of DNA/RNA using PCR you will need the following materials: