AAT Bioquest

What is end-point RT-PCR?

Posted April 24, 2020


RT-PCR (reverse transcription polymerase chain reaction) is a technique used for quantification of RNA, involving reverse transcription of an RNA template into a complementary DNA strand, followed by amplification using PCR. In end-point RT-PCR, PCR products are quantified after total completion of PCR, by staining the products with a fluorescent dye such as ethidium bromide, and then analyzed using gel electrophoresis; in comparison, products are analyzed during every cycle when conducting real-time RT-PCR, through use of fluorescent probes.

Additional resources

6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*

Cyber Green™ [Equivalent to SYBR® Green] *20X Aqueous PCR Solution*

Schmittgen, T. D., Zakrajsek, B. A., Mills, A. G., Gorn, V., Singer, M. J., & Reed, M. W. (2000). Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Analytical biochemistry, 285(2), 194-204. doi:10.1006/abio.2000.4753

Real-Time PCR (qPCR)