logo
AAT Bioquest

What is a common reverse transcriptase?

Posted March 22, 2022


Answer

Reverse transcriptase (RT) is an enzyme used in the reverse transcription process. This enzyme helps to generate complementary DNA (cDNA) from an RNA template. The two common reverse transcriptases are Avian Myeloblastosis Virus Reverse Transcriptase and Moloney Murine Leukemia Virus Reverse Transcriptase.

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT)
Avian Myeloblastosis Virus Reverse Transcriptase is one of the most commonly used reverse transcriptases in many lab assays. It is the preferred transcriptase for reverse transcribing RNAs with strong secondary nature because of its higher reaction temperature. AMV RT is recommended for one-step and two-step RT-PCR and RT-qPCR,
 

Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)
Moloney Murine Leukemia Virus Reverse Transcriptase is often used for long RNAs that are more than >5kb. This is because of its lower RNase H activity compared to AMV RT. The lower reaction temperature of MMLC RT can cause problems when used in the reverse transcription of RNAs with strong secondary structure.

Additional resources

Single-Molecule Analysis of Reverse Transcriptase Enzymes

Aminopropargyl dATP [7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-triphosphate]