logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
What is an appropriate incubation time for primary antibody in western blot analysis?
Posted April 24, 2020
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingGel ElectrophoresisWestern Blotpolyacrylamide gel
Answer

For western blotting, it is generally best to incubate the primary antibody overnight at 4C. Alternatively, incubation for 1-2 hours at room temperature may also be suitable for some antibodies.

Additional resources
Bass, J. J., Wilkinson, D. J., Rankin, D., Phillips, B. E., Szewczyk, N. J., Smith, K., & Atherton, P. J. (2017). An overview of technical considerations for Western blotting applications to physiological research. Scandinavian journal of medicine & science in sports, 27(1), 4-25. doi:10.1111/sms.12702Western Blotting Assays

What is the major difference between Northern and Southern blotting?What is the difference between western blotting and far-western blotting?What can I do if low molecular weight proteins are not showing any bands in western blot?How does a stacking gel work?Can we co-incubate the primary and secondary antibodies at the same time for Western Blotting ?