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What is an appropriate incubation time for primary antibody in western blot analysis?
Posted April 24, 2020
Antibodies and ProteomicsAgarose gel electrophoresisAntibody and Protein LabelingGel ElectrophoresisWestern Blotpolyacrylamide gel
Answer
For western blotting, it is generally best to incubate the primary antibody overnight at 4C. Alternatively, incubation for 1-2 hours at room temperature may also be suitable for some antibodies.
Additional resources
Bass, J. J., Wilkinson, D. J., Rankin, D., Phillips, B. E., Szewczyk, N. J., Smith, K., & Atherton, P. J. (2017). An overview of technical considerations for Western blotting applications to physiological research. Scandinavian journal of medicine & science in sports, 27(1), 4-25. doi:10.1111/sms.12702Western Blotting Assays

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