AAT Bioquest

What is the difference between western blotting and far-western blotting?

Posted April 24, 2020


While western blotting is used to detect proteins, far-western blotting is used to detect protein-protein interactions. This is done by using antibodies for protein detection in western blotting, while using a protein probe in far-western blotting. In western blotting, gel electrophoresis is usually performed under denaturing conditions using SDS, while in far-western blotting, native PAGE (without the use of SDS) may be more beneficial, since it can help to ensure proper protein interactions by maintaining native confirmations.

Additional resources

Mahmood, T., & Yang, P.C. (2012). Western blot: technique, theory, and trouble shooting. North American journal of medical sciences, 4(9), 429-434. doi:10.4103/1947-2714.100998

Wu, Y., Li, Q. & Chen, X. (2007). Detecting protein–protein interactions by far western blotting. Nature Protocols, 2, 3278-3284. doi:10.1038/nprot.2007.459

Walsh, B. W., Lenhart, J. S., Schroeder, J. W., & Simmons, L. A. (2012). Far Western blotting as a rapid and efficient method for detecting interactions between DNA replication and DNA repair proteins. Methods in molecular biology, 922, 161-168. doi:10.1007/978-1-62703-032-8_11

Western Blotting Assays