What is size-exclusion chromatography (SEC) used for in biochemistry?

Posted June 1, 2020

Affinity Chromatography

Chromatographic techniques

Protein Purification

Answer

The main applications of SEC are as follows:

Fractionation of molecules and complexes
Removal of large proteins and complexes
Removal of small molecules such as dyes, primers, and contaminants
Buffer exchange
Desalting
Protein binding studies
Molecular weight determination

Additional resources

ReadiUse™ Bio-Gel P-6 spin column

Amplite™ Rapid Colorimetric Maleimide Quantitation Kit

Hong, P., Koza, S., & Bouvier, E. S. (2012). A review size-exclusion chromatography for the analysis of protein biotherapeutics and their aggregates. Journal of liquid chromatography & related technologies, 35(20), 2923-2950.

Mori, S., & Barth, H. G. (2013). Size exclusion chromatography. Springer Science & Business Media.

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