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What is size-exclusion chromatography (SEC) used for in biochemistry?
Posted June 1, 2020
Answer
The main applications of SEC are as follows:
- Fractionation of molecules and complexes
- Removal of large proteins and complexes
- Removal of small molecules such as dyes, primers, and contaminants
- Buffer exchange
- Desalting
- Protein binding studies
- Molecular weight determination
Additional resources
ReadiUse™ Bio-Gel P-6 spin columnAmplite® Rapid Colorimetric Maleimide Quantitation Kit Hong, P., Koza, S., & Bouvier, E. S. (2012). A review size-exclusion chromatography for the analysis of protein biotherapeutics and their aggregates. Journal of liquid chromatography & related technologies, 35(20), 2923-2950.Mori, S., & Barth, H. G. (2013). Size exclusion chromatography. Springer Science & Business Media.