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What is the actual mechanism involved in blocking a nitrocelluolse membrane ?
Posted April 17, 2020
Antibodies and Proteomics Bioconjugation Chemical ReagentsBSABuffer Preparations and Recipesbovine serum albuminbuffer
Answer
Using a blocking agent such as a buffer containing BSA blocks the non-specific sites on the membrane, thus preventing antibodies from binding to these sites.
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