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Commonly used biological buffers.

Buffer
pH Range
pKa (20 °C)
pKa (25 °C)
pKa (37 °C)
Mol. Wt. (g/mol)
Type
Application
Metal Binding
Recipe
ACES6.1-7.56.886.786.54182.2ZwitterionicCell culture media, protein purification, enzymatic assay (glucosidase activity), x-ray crystallography, yeast, and bacterial studiesStrong: Cu and Mg
Weak: Ca, Mn, Co, Ni, and Zn
ADA6.0-7.26.656.596.46190.155ZwitterionicProtein crystallization, electrophoresis, differential scanning calorimetry, and metal decontamination in soil.Strong: Mn, Co, Ni, Zn, Cd, Pb, and Cu
BES6.4-7.87.177.096.90213.2ZwitterionicCell culture media, chromatography, protein quantification, transfection, bacterial endotoxin studiesStrong: Cu
Weak: Co
Bicine7.6-9.08.358.268.04163.2ZwitterionicCell culture media, chromatography, PCR, protein separationStrong: Fe, Co, Mg, Ca, Ni, Zn, and Cd
Weak: Mn
Bis-Tris5.8-7.2n/a6.506.36209.2ZwitterionicCell culture media, chromatography, electrophoresis, protein purificationStrong: Cu and Pb
Weak: Ca, Cd, Co, Mg, Mn, Ni, and Zn
CAPS9.7-11.110.5610.4010.02221.32ZwitterionicCell culture media, chromatography, electrophoresis, electroblotting, diffusion blottingNegligible
CHES8.6-10.09.559.499.36207.3ZwitterionicCell culture media, chromatography, electron paramagnetic resonance (EPR) spectroscopy, electrophoresis, enzymatic assays, kinetic measurements, yeast dextrose mediumWeak: Cu, Pb, Cd, and Zn
HEPES6.8-8.27.557.45-7.657.31238.3ZwitterionicCell culture media, chromatography, cryopreservation, electrophoresis, electroporation, protein quantificationNegligible
HEPPSO7.1-8.5n/a7.806.66268.33ZwitterionicCell culture media, enzymatic studies, isoelectric focusing, protein quantification, toxicology studiesWeak: Cu
MES5.5-6.76.166.105.97195.2ZwitterionicCell culture media, chromatography, electrochromatography, electrophoresis, fluorescence microscopy, toxicology studies in yeastStrong: Fe
Weak: Cu, Mg, Mn, and Ni
MOPS6.5-7.97.287.207.02209.3ZwitterionicCell culture media, chromatography, electrophoresis, protein purification and quantification, lysis buffer, toxicology studies in yeastStrong: Fe
Weak: Mg, Mn, Co, and Ni
MOPSO6.2-7.6n/a6.906.75225.3ZwitterionicCell culture media, electrochromatography, electrophoresis, enzyme assays, fluorescence spectroscopy, isothermal titration calorimetry, lysis buffer, spectrophotometryStrong: Fe
Weak: Ni
PIPES6.1-7.56.806.766.66302.37ZwitterionicCell culture media, chromatography, protein purificationNegligible
TAPS7.7-9.18.498.408.18243.28ZwitterionicCell culture media, chromatography, DNA analysis, enzymatic assay (aminopeptidase activity),Strong: Cu, Cr, and Fe
Weak: Zn, Cd, and Pb
n/a
TES6.8-8.27.507.407.16229.25ZwitterionicCell culture media, chromatography, DNA precipitation and extraction, enzyme assays, protein quantificationStrong: Cu, Cr, and Fe
Weak: Co, Ni, Cu, and Zn
Tricine7.4-8.88.168.057.80179.2ZwitterionicCell culture media, electrophoresisStrong: Ca, Co, Cu, Mg, Ni, and Zn
Weak: Mn
Tris7.0-9.08.208.067.72121.14ZwitterionicElectrophoresis, anion exchange chromatography, capillary electrochromatography, buffer for vaccinesStrong: Co, Cu, Cr, Fe, and Ni
Weak: Ca, Cd, Mg, Pb, and Zn



References

This online tool may be cited as follows

MLA

"Quest Database™ Commonly used biological buffers.." AAT Bioquest, Inc.20 Jun2024https://www.aatbio.com/data-sets/commonly-used-biological-buffers.

APA

AAT Bioquest, Inc. (2024June 20). Quest Database™ Commonly used biological buffers.. AAT Bioquest. https://www.aatbio.com/data-sets/commonly-used-biological-buffers.
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