Products
Technologies
Applications
Services
Resources
Selection Guides
About
Commonly used biological buffers.
| Buffer | pH Range | pKa (20 °C) | pKa (25 °C) | pKa (37 °C) | Mol. Wt. (g/mol) | Type | Application | Metal Binding | Recipe |
|---|---|---|---|---|---|---|---|---|---|
| ACES | 6.1-7.5 | 6.88 | 6.78 | 6.54 | 182.2 | Zwitterionic | Cell culture media, protein purification, enzymatic assay (glucosidase activity), x-ray crystallography, yeast, and bacterial studies | Strong: Cu and Mg Weak: Ca, Mn, Co, Ni, and Zn |  |
| ADA | 6.0-7.2 | 6.65 | 6.59 | 6.46 | 190.155 | Zwitterionic | Protein crystallization, electrophoresis, differential scanning calorimetry, and metal decontamination in soil. | Strong: Mn, Co, Ni, Zn, Cd, Pb, and Cu | |
| BES | 6.4-7.8 | 7.17 | 7.09 | 6.90 | 213.2 | Zwitterionic | Cell culture media, chromatography, protein quantification, transfection, bacterial endotoxin studies | Strong: Cu Weak: Co | |
| Bicine | 7.6-9.0 | 8.35 | 8.26 | 8.04 | 163.2 | Zwitterionic | Cell culture media, chromatography, PCR, protein separation | Strong: Fe, Co, Mg, Ca, Ni, Zn, and Cd Weak: Mn | |
| Bis-Tris | 5.8-7.2 | n/a | 6.50 | 6.36 | 209.2 | Zwitterionic | Cell culture media, chromatography, electrophoresis, protein purification | Strong: Cu and Pb Weak: Ca, Cd, Co, Mg, Mn, Ni, and Zn | |
References
This online resource may be cited as follows
MLA | "Quest Database™ Commonly used biological buffers.." AAT Bioquest, Inc., 28 Sep. 2025, https://www.aatbio.com/data-sets/commonly-used-biological-buffers. | |
APA | AAT Bioquest, Inc. (2025, September 28). Quest Database™ Commonly used biological buffers.. AAT Bioquest. https://www.aatbio.com/data-sets/commonly-used-biological-buffers. | |
| BibTeX | EndNote | RefMan |