AAT Bioquest

What is the best concentration to use with Hoechst?

Posted May 6, 2021


Hoechst can be used at concentrations of 1-10 ug/mL in phosphate buffered saline (PBS) or any other detergent-free buffer at room temperature. The best concentration for staining live cells with Hoechst is 1 ug/mL. Cytotoxicity is minimum at this concentration, ensuring a higher viability of stained live cells. Over-staining with Hoechst is very rare because the dye has limited binding sites. 

Additional resources

Spectroscopic study of interaction of various GC-content DNA with Hoechst 33258 depending on Na + concentration

Hoechst 33258 *CAS 23491-45-4*

Hoechst 33342 *Ultrapure Grade*