AAT Bioquest

What is the difference between RT-PCR and RNA Seq?

Posted March 2, 2022


RT-PCR or Reverse Transcriptase PCR is a technique used to generate multiple copies of a genetic sequence of interest for analysis. Researchers use RT-PCR to study gene expression and measure expression levels. It is a vital tool in diagnosing genetic diseases and monitoring drug therapy.

RNA-seq or RNA-sequencing is a technique used to determine the quantity and exact sequence of RNA in a sample. Researchers use RNA-seq to determine which genes are expressed in different types of cells as well as when and how these genes are expressed. It is a vital tool in learning more about various aspects of cancer diagnosis and therapy.

Additional resources

Comprehensive molecular screening: from the RT-PCR to the RNA-seq

MagaDye™ 4 Color Sanger Sequencing Terminator Kit