What is the difference between Zymography and SDS-PAGE?
Posted April 24, 2020
The purposes of SDS-PAGE and zymography are substantially different—while SDS-PAGE is largely used to determine the molecular weight of proteins, zymography is used to determine enzymatic activity. In addition, proteins are fully denatured when performing SDS-PAGE, but with zymography, proteins undergo renaturation due to the absence of any reducing agents, and in that way retain enzymatic activity.
Hu, X., & Beeton, C. (2010). Detection of Functional Matrix Metalloproteinases by Zymography. Journal of Visualized Experiments, (45), e2445. doi:10.3791/2445
Leber, T.M., & Balkwill F.R. (1997). Zymography: A Single-Step Staining Method for Quantitation of Proteolytic Activity on Substrate Gels. Analytical Biochemistry. 249(1), 24-28. doi:10.1006/abio.1997.2170