AAT Bioquest

Where do reactive oxygen species (ROS) come from?

Posted May 18, 2020


The ROS can be produced from either endogenous or exogenous sources. The endogenous sources of ROS include different cellular organs such as mitochondria, peroxisomes, and endoplasmic reticulum, where the oxygen consumption is high. Phagocytic cells such as neutrophils, eosinophils, and mononuclear phagocytes can also produce ROS when stimulated. On the other hand, ROS are also generated in the biological systems by various exogenous source, such as pollution, alcohol, tobacco smoke, heavy metals, transition metals, industrial solvents, pesticides, certain drugs like halothane, paracetamol, and radiation.

Additional resources

Reactive Oxygen Species (ROS)

ROS Detection: ROS probe selection guide

Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD (October 2004). Free radicals and antioxidants in human health: current status and future prospects. The Journal of the Association of Physicians of India. 52: 794–804. PMID 15909857

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*