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Endoplasmic Reticulum (ER)

The endoplasmic reticulum (ER) is an organelle present in most eukaryotic cells. It is primarily responsible for the synthesis and transport of cellular products such as proteins, lipids and hormones. Structurally, the ER is comprised of an interconnected network of flattened, membrane-enclosed sacs and tubules that extend from the nuclear membrane throughout the cytoplasm. These sac-like structure and tubules are held together and supported by the cytoskeleton.

The ER complex is comprised of two subunits - rough ER and smooth SER. The surface of the rough ER is coated with protein-manufacturing ribosomes. Once synthesized, membrane-bound transport vesicles shuttle these proteins to the Golgi apparatus. The smooth ER lacks ribosomes and is responsible for the synthesis of lipids, phospholipids and steroids. Secondary responsibilities of the smooth ER include carbohydrate and steroid metabolism, detoxification and modulation of calcium ions.

 

 

Endoplasmic Reticulum Staining Kits for Live Cells


ER Tracer™ dyes are membrane-permeant stains considerably selective for the endoplasmic reticulum in living cells. As the key component in our Cell Navigator® Live Cell Endoplasmic Reticulum Staining Kits, ER Tracer™ dyes can be multiplexed with other fluorescent proteins or probes in live cell multiparametric studies or after fixation for colocalization studies. However, for certain cell lines, ER Tracer™ stains may not selectively bind to ER.

Key Features of Cell Navigator® Live Cell ER Staining Kits:

  • Highly-selective for ER over other cellular compartments (e.g. lysosomes and mitochondria)
  • ER staining well-retained after cell fixation
  • Available in blue, green and red fluorescence to facilitate multiplexing with other fluorescent probes
  • Suitable for proliferating and non-proliferating cells
  • Kits includes a robust staining protocol and sufficient materials for 100 tests


ER of HeLa cells labeled using Cell Navigator® Live Cell Endoplasmic Reticulum Staining Kits. ER, nuclei, mitochondria or F-actin were stained, respectively with (A) Cell Navigator® Live Cell Endoplasmic Reticulum Staining Kit *Blue Fluorescence* (Cat No. 22634) and Nuclear Red™ LCS1 (Cat No. 17542), (B) Cell Navigator® Live Cell Endoplasmic Reticulum Staining Kit *Green Fluorescence* (Cat No. 22635), DAPI (Cat No. 17507) and Phalloidin-iFluor® 594 (Cat No. 23122), and (C) Cell Navigator® Live Cell Endoplasmic Reticulum Staining Kit *Red Fluorescence* (Cat No. 22636), MitoLite™ Green (Cat No. 22675) and Hoechst 33342 (Cat No. 17535).

 

Table 1. Cell Navigator® ER Staining Kits for imaging the endoplasmic reticulum of live cells.

Kit
Probe
Ex/Em (nm)
Filter Set
Unit Size
Cat No.
Cell Navigator® Live Cell ER Staining Kit *Blue Fluorescence*ER Tracer™ Blue344/456DAPI100 Tests22634
Cell Navigator® Live Cell ER Staining Kit *Green Fluorescence*ER Tracer™ Green503/511FITC100 Tests22635
Cell Navigator® Live Cell ER Staining Kit *Red Fluorescence*ER Tracer™ Red589/620Texas Red100 Tests22636

 

Product ordering information


 

Table 2. Ordering Info for Cell Navigator Live Cell ER Satining Kits Products

Cat#
Product Name
Unit Size
Price
22634Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence*100 Tests310
22635Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Green Fluorescence*100 Tests310
22636Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence*100 Tests310