ROS Detection: ROS probe selection guide.

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Accepted Answer

Table 1. Intracellular reactive oxygen species (ROS) detection reagents and assay kits

ROS Species
ROS Brite&trade; 570
ROS Brite&trade; 670
ROS Brite&trade; 700
ROS Brite&trade; HDCF
Amplite® ROS Green
Amplite® ROS Red

Hydrogen Peroxide (H2O2)
+
+
+
+++
+++
+++

Hydroxyl radical (&bull;OH)
++
++
++
+
+
+

Tert-butyl-hyrdoperoxide (TBHP)
+
+
+
+
+
+

Hypochlorous acid (HOCl)
-
+
++
-
+
-

Superoxide anion (O2&bull;-)
+
++
++
-
-
-

Nitric Oxide (NO)
-
-
-
-
-
-

Peroxynitrite anion (ONOO-)
-
-
-
-
-
-

Cat No.
22902
22903
16004
16053

22900
22904

22901

Table 2. Selective reactive oxygen species (ROS) detection reagents and assay kits.

ROS Species
OxiVision&trade; Green
OxiVision&trade; Blue
MitoROS&trade; 520
MitoROS&trade; 580
MitoROS&trade; OH580

Hydrogen Peroxide (H2O2)
+++
+++
-
-
-

Hydroxyl radical (&bull;OH)
-
-
-
-
+++

Tert-butyl-hyrdoperoxide (TBHP)
-
-
-
-
-

Hypochlorous acid (HOCl)
-
-
-
-
-

Superoxide anion (O2&bull;-)
-
-
+++
+++
-

Nitric Oxide (NO)
-
-
-
-
-

Peroxynitrite anion (ONOO-)
-
-
-
-
-

Cat No.

11503
11506

11504
11505

16060

22970
22971

16055

ROS Species	ROS Brite™ 570	ROS Brite™ 670	ROS Brite™ 700	ROS Brite™ HDCF	Amplite® ROS Green	Amplite® ROS Red
Hydrogen Peroxide (H₂O₂)	+	+	+	+++	+++	+++
Hydroxyl radical (•OH)	++	++	++	+	+	+
Tert-butyl-hyrdoperoxide (TBHP)	+	+	+	+	+	+
Hypochlorous acid (HOCl)	-	+	++	-	+	-
Superoxide anion (O₂^•-)	+	++	++	-	-	-
Nitric Oxide (NO)	-	-	-	-	-	-
Peroxynitrite anion (ONOO^-)	-	-	-	-	-	-
Cat No.	22902	22903	16004	16053	22900 22904	22901

ROS Species	OxiVision™ Green	OxiVision™ Blue	MitoROS™ 520	MitoROS™ 580	MitoROS™ OH580
Hydrogen Peroxide (H₂O₂)	+++	+++	-	-	-
Hydroxyl radical (•OH)	-	-	-	-	+++
Tert-butyl-hyrdoperoxide (TBHP)	-	-	-	-	-
Hypochlorous acid (HOCl)	-	-	-	-	-
Superoxide anion (O₂^•-)	-	-	+++	+++	-
Nitric Oxide (NO)	-	-	-	-	-
Peroxynitrite anion (ONOO^-)	-	-	-	-	-
Cat No.	11503 11506	11504 11505	16060	22970 22971	16055