What is the working mechanism of MitoROS 580?

MitoROS™ 580 is a cell-permeant, superoxide-sensitive probe designed to monitor superoxide production in mitochondria using a fluorescence microscope or flow cytometer. Upon entering live cells, the non-fluorescent MitoROS™ 580 is rapidly oxidized by superoxide. The oxidized product is highly fluorescent upon binding to nucleic acid.

Key Features of MitoROS™ 580 include:

• Cell-permeable superoxide probe designed for live cell imaging
• Readily oxidized by superoxide but not by other reactive oxygen species (ROS) and reactive nitrogen species (RNS)
• Excitation/Emission maxima of approximately 510/580 nm