AAT Bioquest

What are some ROS probes?

Posted May 14, 2019

What are the differences between APF, HPF and DCFH-DA?


APF (Cat# 16050) is an ROS probe specific to hydroxyl radical (●OH), peroxynitrite anion (ONOO) and hypochlorite anion (ClO).

HPF (Cat# 16051) is an ROS probe specific to ●OH and ONOO.

DCFH-DA (Cat# 15204) is an ROS probe responsive to all ROS species and is often used as a total ROS probe.

APF and HPF are more stable and have low auto-oxidation effect compared to DCFH-DA, which is easily oxidized. We have observed a gradual increase in fluorescence over time in DCFH-DA stained cells that are exposed to excitation light under microscope. 

Additional resources

ROS Brite™ APF Product Page

ROS Brite™ HPF Product Page

DCFH-DA Product Page