What is the function of primers in DNA PCR process?
Posted September 25, 2020
Primers are short, single-stranded DNA sequences that are used in PCR as a starting point for DNA synthesis. During the annealing step, PCR primers hybridize to a specific location on the single-stranded DNA template via complementary base pairing. Once bound, DNA polymerases attach to each primer and begin incorporating nucleotides along the template strand in the 5’ to 3’ direction.
PCR Detection of Viral DNA/RNA
Cyber Green™ [Equivalent to SYBR® Green] *20X Aqueous PCR Solution*