What is the purpose of affinity chromatography?
Posted December 15, 2021
Answer
Affinity chromatography is used to separate a protein of interest from other proteins based on its interaction with a specific ligand. Examples include interactions between enzyme/substrate, antibody/antigen or enzyme/inhibitor. This technique utilizes a protein’s biological structure or function for purification, which results in higher resolution, selectivity, and capacity in protein purification studies.
Additional resources
Affinity chromatography: A versatile technique for antibody purification