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What is the right concentration of antibodies to use when labelling with fluorescent dyes?
Posted April 24, 2020
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)
Answer

It is generally advisable to use antibody concentrations of at least 1 mg/ml for optimal labeling. Antibody concentrations lower than 1 mg/ml can result in reduced conjugation efficiency.

The amount of dye needed is dependent on the desired dye-to-antibody ratio.

Additional resources
ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

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