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What kind of excitation light does Hoechst require?
Posted May 6, 2021
NucleusDNA and RNABioconjugation
Answer

Hoechst dyes are excited by ultraviolet light (~360 nm) of xenon lamps, mercury-arc lamps or UV lasers. When exposed to the right excitation light, Hoechst dyes emit blue fluorescent light around an emission spectrum range of 460 to 490 nm.

Additional resources
Side population analysis using a violet-excited cell-permeable DNA binding dyeHoechst 33258 *CAS 23491-45-4*Hoechst 33342 *Ultrapure Grade*

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