What kind of excitation light does Hoechst require?
Posted May 6, 2021
Hoechst dyes are excited by ultraviolet light (~360 nm) of xenon lamps, mercury-arc lamps or UV lasers. When exposed to the right excitation light, Hoechst dyes emit blue fluorescent light around an emission spectrum range of 460 to 490 nm.
Side population analysis using a violet-excited cell-permeable DNA binding dye
Hoechst 33258 *CAS 23491-45-4*