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What makes a good fluorophore?
Posted March 16, 2021
Classic DyesChemical Reagents
Answer

A good fluorophore is one that has a considerable separation between the excitation and emission maxima. The larger difference in the separation makes the detection more reliable as compared to a fluorophore with very little separation.

Additional resources
Evaluation of fluorophores for optimal performance in localization-based super-resolution imagingCyanine 5 azide [equivalent to Cy5® azide]Classic Dyes

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