What phagocytic particles are best for assaying phagocytic activity of macrophages?
Posted May 9, 2020
Latex beads, zymosan, and E. coli are a few of the most commonly used particles for phagocytosis assays. Latex beads and zymosan can be labeled with a dye such as FITC, while E. coli can be made to express GFP. These particles can also be opsonized with IgG if necessary.
Havixbeck, J. J., Rieger, A. M., Wong, M. E., Wilkie, M. P., & Barreda, D. R. (2014). Evolutionary conservation of divergent pro-inflammatory and homeostatic responses in Lamprey phagocytes. PloS one, 9(1).