AAT Bioquest

How to quench glutaraldehyde fluorescence?

Posted April 17, 2020


The aldehyde functional group of glutaraldehyde allows it to act as a preservative by binding to amine groups and ultimately keeping proteins in a cell intact. However, this process causes autofluorescence due to the binding of glutaraldehyde to labeled antibodies. In order to quench this fluorescence, the aldehyde groups must be reduced so that the binding does not occur. To do this, a reducing agent such as sodium borohydride or Schiff’s reagent can be used.