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Which hydrogen peroxide assays can I use if NADH is present in my sample?
Posted June 28, 2018
Which of your hydrogen peroxide (H2O2) assay kits (Cat# 11501 and 11502) should I use to avoid any interference with NADH? I have around 50-100 µM of NADH in my sample.
Answer
We tested both of our Amplite® Fluorimetric Hydrogen Peroxide Assay Kits using samples containing 80 µM of NADH. NADH was dissolved in PBS, Tris buffer, HH buffer and other phosphate buffers with a pH ranging from 7-8. Under various different conditions, Cat# 11502 showed zero interference with NADH when detecting H2O2. For your experiment, we recommend Cat# 11502.