Which ROS reagent is best for use in small animals such as mice or rats? What is the recommended dosage?
Posted June 13, 2019
Can you provide a sample protocol and any publications?
We recommend using ROS Brite™ 700 (Cat# 16004) for in vivo imaging in small animals.
The concentration of ROS Brite™ 700 required varies between different cell lines, we recommend testing with different concentrations to determine the optimal dosage. Foersch and colleagues used ROS Brite™ 700 for in vivo imaging in mice (Foersch et al. 2015). They administered a final concentration of 10 μg/g body weight, intravenously, and waited 20 minutes before examining.
For a sample protocol and additional publications using ROS Brite™ 700, see links below in Resources.