Products
Services
Resources
Selection Guides
About
Will reducing agents cause any interference when using your hydrogen peroxide assays?
Posted May 20, 2019
I have redox cycling compounds that generate H2O2 when in the presence of a strong reducing agent like DTT or TCEP. I need a fluorimetric hydrogen peroxide assay than can tolerate μM concentrations of DTT or TCEP. Can your H2O2 assays tolerate DTT or TCEP and at what concentrations?
Answer
We offer two H2O2 assays:
- Amplite® Fluorimetric Hydrogen Peroxide Assay Kit Red Fluorescence (Cat# 11501)
- Amplite® Fluorimetric Hydrogen Peroxide Assay Kit Near Infrared Fluorescence (Cat# 11502)
For both kits, DTT increases signal intensity. It will not affect the detection of H2O2 if the concentration of DTT is lower than 10 μM.
For Cat# 11501, TCEP will not affect the detection of H2O2 if the concentration of TCEP is lower than 10 μM. For Cat# 11502, 10 μM of TCEP decreases signal intensity thus allowing for the detection of H2O2 at concentrations above 1 μM. The assay sensitivity will become lower, therefore when using Cat# 11502 we recommend having no more than 1 μM of TCEP in your system.