Will reducing agents cause any interference when using your hydrogen peroxide assays?

I have redox cycling compounds that generate H₂O₂ when in the presence of a strong reducing agent like DTT or TCEP. I need a fluorimetric hydrogen peroxide assay than can tolerate μM concentrations of DTT or TCEP. Can your H₂O₂ assays tolerate DTT or TCEP and at what concentrations?

We offer two H₂O₂ assays: 

• Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence* (Cat# 11501)
• Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Near Infrared Fluorescence* (Cat# 11502)

For both kits, DTT increases signal intensity. It will not affect the detection of H₂O₂ if the concentration of DTT is lower than 10 μM. 

For Cat# 11501, TCEP will not affect the detection of H₂O₂ if the concentration of TCEP is lower than 10 μM. For Cat# 11502, 10 μM of TCEP decreases signal intensity thus allowing for the detection of H₂O₂ at concentrations above 1 μM. The assay sensitivity will become lower, therefore when using Cat# 11502 we recommend having no more than 1 μM of TCEP in your system.