I have redox cycling compounds that generate H2O2 when in the presence of a strong reducing agent like DTT or TCEP. I need a fluorimetric hydrogen peroxide assay than can tolerate µM concentrations of DTT or TCEP. Can your H2O2 assays tolerate DTT or TCEP and at what concentrations?
We offer two H2O2 assays:
- Amplite® Fluorimetric Hydrogen Peroxide Assay Kit Red Fluorescence (Cat# 11501)
- Amplite® Fluorimetric Hydrogen Peroxide Assay Kit Near Infrared Fluorescence (Cat# 11502)
For both kits, DTT increases signal intensity. It will not affect the detection of H2O2 if the concentration of DTT is lower than 10 µM.
For Cat# 11501, TCEP will not affect the detection of H2O2 if the concentration of TCEP is lower than 10 µM. For Cat# 11502, 10 µM of TCEP decreases signal intensity thus allowing for the detection of H2O2 at concentrations above 1 µM. The assay sensitivity will become lower, therefore when using Cat# 11502 we recommend having no more than 1 µM of TCEP in your system.