Why do we use Citrate buffer in Antigen retrieval step during IHC? | AAT Bioquest

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Why do we use Citrate buffer in Antigen retrieval step during IHC?

Posted April 17, 2020

Antibodies and Proteomics Antibody and Protein Labeling Immunohistochemistry (IHC)buffer Chemical Reagents Buffer Preparations and Recipes

Answer

Citrate buffer is used in the antigen retrieval step during IHC in order to break formalin cross links that formed during fixation. By boiling in hot citrate buffer, the antigen is able to be unmasked.

Additional resources

Immunohistochemistry (IHC)

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