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Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Posted April 17, 2020
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent
Answer
In general, it is best to use Triton X-100 prior to incubation with the primary antibody and exclude the Triton X-100 during primary antibody incubation. This is because Triton X-100 is a harsh detergent. If used during primary antibody incubation, it has the potential to remove epitopes from tissue, which will reduce the signal intensity.