AAT Bioquest

Why is 100% Ethanol used in rehydration of paraffin embedded tissues?

Posted April 17, 2020


In the rehydration of paraffin embedded tissues, it is important to immerse the slides in 100% ethanol after treating with xylene because xylene is immiscible with water. The slides cannot be immediately changed from xylene to the subsequent ethanol solutions containing water or else xylene may remain on the tissue section and interfere with staining. Since ethanol is miscible with both xylene and water, 100% ethanol effectively clears xylene and prepares the samples for introduction of water.

Additional resources

Immunohistochemistry (IHC)

Paraffin-Embedded Tissue Immunohistochemistry