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AAT Bioquest

Why is NaCl used in size exclusion chromatography?

Posted March 18, 2022


Answer

Size exclusion chromatography (SEC) is a laboratory technique that separates proteins according to their hydrodynamic radius (size). NaCl is used in this process to minimize secondary interactions between the sample components and the resin.

Additional resources

Gel filtration chromatography (size exclusion chromatography) of proteins

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