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AAT Bioquest

Why is PCR used in the process of DNA sequencing?

Posted March 11, 2022


Answer

PCR (Polymerase Chain Reaction) is a process used to make many copies of a specific DNA region in vitro. This technique can produce millions or even billions of copies of the target DNA region very quickly.

The goal of using PCR is to produce enough copies of the target DNA region. It is particularly useful in the process of DNA sequencing when the DNA sample is too small such as the sample obtained in crime scene evidence.  

Using PCR in the process of DNA sequencing has a number of research and practical applications in genetic testing, forensics, and medical diagnostics. All genetic and molecular analyses applications require significant amounts of DNA samples. If the DNA sample is too small, PCR amplification can be used to generate millions of copies of the target DNA region.

Additional resources

PCR and DNA sequencing

Helixyte™ Green *10,000X Aqueous PCR Solution*