Can DAPI bind to RNA?
Posted December 10, 2022
DAPI can bind to RNA, although with a lower affinity than it does for DNA and also emits at a longer wavelength (500 nm). The dye intercalates through AU-selective intercalation, and only yields a 20% increase in quantum yield as compared to the DAPI/dsDNA complex. DAPI binds to both RNA and DNA at the minor groove of A-T rich regions.