Can glycerol be used in SDS-PAGE? | AAT Bioquest

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Can glycerol be used in SDS-PAGE?

Posted July 17, 2024

Chemical Reagents DNA and RNA Quantitation Gel Electrophoresis Nucleic Acids

Answer

Yes, Glycerol can be used in SDS-PAGE. Glycerol is added to the loading buffer to increase density of the sample, so it does not diffuse out of the gel.

Additional resources

Effect of glycerol on the separation of nucleosomes and bent DNA in low ionic strength polyacrylamide gel electrophoresis Gel Electrophoresis SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe Gel Electrophoresis Annotator

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