The steps needed to make a phosphate buffer are listed in numerical order below. It is important to note that this is the procedure for a phosphate buffer of 5.8 to 7.4.
Prepare 800 mL of distilled water in the appropriate container
Add 20.214 g of Sodium Phosphate Dibasic Heptahydrate to the solution
Add 3.394 g of Sodium Phosphate Monobasic Monohydrate to the solution
Alter the solution to the final desired pH using HCL or NaOH