How does CRISPR-Cpf1 differ from CRISPR-Cas9?

Question

Accepted Answer

Basis of differentiation

CRISPR-Cpf1

CRISPR-Cas9

Definition

CRISPR-associated protein Cpf1, a genome editing tool that differs from Cas9

CRISPR-associated protein 9, a genome editing tool derived from CRISPR systems

Enzyme Size

Smaller, facilitating easier delivery into cells and tissues

Larger

RNA Requirement

Requires only a single RNA for cutting activity

Requires two small RNAs for cutting

Cutting location

Cuts DNA far away from recognition site

Cuts DNA closer to recognition site, allowing multiple opportunities for correct editing

PAM Recognition

Recognizes different PAM sequences compared to Cas9, providing new flexibility in target selection

Recognizes specific PAM sequences

Cutting Mechanisms

Cuts DNA strands at offset positions, leaving short overhangs

Cuts both DNA strands at the same place, creating blunt ends

Basis of differentiation	CRISPR-Cpf1	CRISPR-Cas9
Definition	CRISPR-associated protein Cpf1, a genome editing tool that differs from Cas9	CRISPR-associated protein 9, a genome editing tool derived from CRISPR systems
Enzyme Size	Smaller, facilitating easier delivery into cells and tissues	Larger
RNA Requirement	Requires only a single RNA for cutting activity	Requires two small RNAs for cutting
Cutting location	Cuts DNA far away from recognition site	Cuts DNA closer to recognition site, allowing multiple opportunities for correct editing
PAM Recognition	Recognizes different PAM sequences compared to Cas9, providing new flexibility in target selection	Recognizes specific PAM sequences
Cutting Mechanisms	Cuts DNA strands at offset positions, leaving short overhangs	Cuts both DNA strands at the same place, creating blunt ends