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AAT Bioquest

How does mRNA work?

Posted February 20, 2023


Answer

mRNA, or messenger RNA, is first synthesized in the nucleus by using the nucleotide sequence of DNA as its template. This process requires nucleotide triphosphates as substrates and is catalyzed by RNA polymerase II. During transcription, mRNA regulates the synthesis of proteins, which occurs in the cytoplasm. Proteins are assembled on the ribosomes using mRNA nucleotides as instructions. mRNA carries information from the nucleus to the cytoplasm. The information is encoded to the nucleotide sequence of the mRNA. The creation of proteins from mRNA is a process known as translation.      

Additional resources

mRNA

DNA and RNA Quantitation

Portelite™ Fluorimetric RNA Quantitation Kit*Optimized for Cytocite™ and Qubit™ Fluorometers*

Transfectamine™ mRNA Transfection Reagent