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How does reverse transcriptase work?
Posted February 23, 2023
DNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)
Answer

Reverse transcriptase, an enzyme found mainly in retroviruses, works by converting the RNA genome carried in the retrovirus particle into cDNA (complementary DNA) sequences that are capable of inserting into various areas of the genome.

Additional resources
Reverse transcriptase kinetics for one-step RT-PCR DNA and RNA Quantitation Reverse Transcription PCR (RT-PCR) ReadiScript™ RT Reverse Transcription Kit

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