AAT Bioquest

How does trypan blue distinguish living and dead cells?

Posted November 16, 2022


Trypan blue distinguishes between live and dead cells by using a hemocytometer and microscope. The process is this; after your sample of cells are suspended and treated with trypan blue, you then put sample onto a hemocytometer slide. Then, you must view the cells under a microscope under 10X power objective. Here, you can see which cells have been stained, and which have not. Like its name implies trypan blue stains the dead cells blue, so you can easily distinguish between live and dead cells. 

Additional resources

Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*

Cell Viability Assays

Trypan Blue Dye Enters Viable Cells Incubated with the Pore-Forming Toxin HlyII of Bacillus cereus