How does tyramide amplification work?
Posted May 10, 2024
Answer
Tyramide signal amplification works by using horseradish peroxidase (HRP) to convert an inactive form of tyramide, which is labeled with a fluorophore or a chemical marker like biotin, into an active form. This active tyramide binds covalently to tyrosine residues on proteins near the HRP. By targeting the HRP to specific molecules of interest using antibodies, the technique achieves high-density labeling at the target site. Thus, it makes it easier to detect even small amounts of biomolecules. Detection can be direct, using fluorescence, or indirect, using biotin.
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