Name: iFluor® 488 tyramide
Brand: AAT Bioquest
SKU: 45100
Price: 355 USD
Availability: InStock

iFluor® 488 tyramide

iFluor® 488 Tyramide is a green-fluorescent signal amplification reagent for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Tyramide Signal Amplification: Enhances detection sensitivity through HRP-catalyzed fluorescence amplification.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
HRP-Mediated Labeling: Enables localized covalent deposition of fluorescent labels near target sites.
Filter Compatibility: Supports fluorescence imaging using standard FITC filter sets.
Applications: Suitable for amplified immunofluorescence and immunohistochemical staining of cells and tissues.

Immunofluorescent image of paraffin-embedded human lung carcinoma labeled with  EpCAM Rabbit mAb followed with HRP-labeled goat anti-rabbit IgG (H+L) (Cat#16793) . The signal was developed with iFluor® 488 tyramide or Alexa Fluor™ 488 Tyramide Reagent (Green). Cells were also counterstained with DAPI (Blue).

Protocol

Catalog	Size	Price	Quantity
45100	200 slides	Price

Overview

For many immunohistochemical (IHC) applications, traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit their sensitivity and utility. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label results in the ability to detect low-abundance targets with ultrasensitive precision and reduces the amount of antibodies and hybridization probes needed. In IHC applications, this method can also enhance sensitivity in cases where the primary antibody dilution needs to be increased to reduce nonspecific background signals or overcome weak immunolabeling due to suboptimal fixation procedures or low levels of target expression. The iFluor® 488 tyramide contains the bright iFluor® 488 that can be readily detected with the standard FITC filter set. iFluor® dyes have higher florescence intensity, increased photostability, and enhanced water solubility, resulting in fluorescence signals with significantly higher precision and sensitivity. iFluor® 488 is an excellent replacement for Alexa Fluor® 488 tyramide (Alexa Fluor® is the trademark of ThermoFisher), FITC tyramide, or other comparable fluorescent tyramide conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	188.157 µL	940.787 µL	1.882 mL	9.408 mL	18.816 mL
5 mM	37.631 µL	188.157 µL	376.315 µL	1.882 mL	3.763 mL
10 mM	18.816 µL	94.079 µL	188.157 µL	940.787 µL	1.882 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	531.47 g/mol	=	mL	x	mM	=	mmol

Citations

View all 45 citations: Citation Explorer

Microbiota-mediated naringenin release limits postoperative acute gastrointestinal injury by suppressing ferroptosis

Authors:

Deng, Fan and Hu, Jingjuan and Min, Yue and Xie, Sun and Lin, Zebin and Sun, Qishun and Liu, Huamin and He, Shiying and Feng, Siyuan and Wu, Ling and others,

Journal:

Cell Reports Medicine (2026)

A lipoprotein-biomimetic nanoplatform for oncogenic KRAS mutation-specific lysosome-targeting protein degradation

Authors:

Yang, Hang and Zhou, Xinyi and Wang, Jiafeng and Yang, Chenyue and Jin, Yuzhao and Liao, Xufeng and Liu, Xiangrui and Guo, Chuansheng and Xiao, Qian and Zhou, Quan and others,

Journal:

Nature Communications (2026)

Echinocystic acid alleviates cerebral ischemia-reperfusion injury through PPAR$\gamma$-dependent modulation of microglial polarization and pyroptotic signaling

Authors:

Zou, Yi-Kai and Zhang, Hui and Liu, Jian-Xia and He, Shu-Yi and Yang, Jiao-Yang and Peng, Hai-Jing and Zhang, Jia-Xi and Sun, Jing and Min, Jia-Wei

Journal:

International Immunopharmacology (2026): 117392

CD46 and DSG2 synergistically mediate human adenovirus type 7 infection

Authors:

Ye, Lihua and Mo, Chuncong and Yuan, Jinwei and Su, Yalin and Zhou, Chengxing and Yang, Yujie and Li, Xiao and Liu, Wenkuan and Zeng, Rongjie and Zhou, Rong and others,

Journal:

Journal of Virology (2026): e00136--26

Analysis of paired primary-metastatic renal cancer lesions reveals RCN3-MMP10 axis facilitates lung metastasis

Authors:

Lin, Wenhao and Wang, Qi and Xu, Kandi and Ding, Jiawei and Ma, Zhiyang and Xing, Siwei and Xu, Danfeng and He, Yayi and Chen, Lu

Journal:

Cellular Signalling (2026): 112534

References

View all 74 references: Citation Explorer

Tyramide Signal Amplification for Immunofluorescent Enhancement

Authors:

Faget L, Hnasko TS.

Journal:

Methods Mol Biol (2015): 161

Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification

Authors:

Trang NT, Hirai T, Ngan PH, Lan NT, Fuke N, Toyama K, Yamamoto T, Yamaguchi R.

Journal:

J Vet Diagn Invest (2015): 326

Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis

Authors:

Stack EC, Wang C, Roman KA, Hoyt CC.

Journal:

Methods (2014): 46

Sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification

Authors:

Lauter G, Soll I, Hauptmann G.

Journal:

Methods Mol Biol (2014): 175

KSHV cell attachment sites revealed by ultra sensitive tyramide signal amplification (TSA) localize to membrane microdomains that are up-regulated on mitotic cells

Authors:

Garrigues HJ, Rubinchikova YE, Rose TM.

Journal:

Virology (2014): 75

Physical properties

Molecular weight	531.47
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 28, 2026