Name: iFluor® 488 tyramide
Brand: AAT Bioquest
SKU: 45100
Price: 348 USD
Availability: InStock

iFluor® 488 tyramide

For many immunohistochemical (IHC) applications, traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit their sensitivity and utility. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label results in the ability to detect low-abundance targets with ultrasensitive precision and reduces the amount of antibodies and hybridization probes needed. In IHC applications, this method can also enhance sensitivity in cases where the primary antibody dilution needs to be increased to reduce nonspecific background signals or overcome weak immunolabeling due to suboptimal fixation procedures or low levels of target expression. The iFluor® 488 tyramide contains the bright iFluor® 488 that can be readily detected with the standard FITC filter set. iFluor® dyes have higher florescence intensity, increased photostability, and enhanced water solubility, resulting in fluorescence signals with significantly higher precision and sensitivity. iFluor® 488 is an excellent replacement for Alexa Fluor® 488 tyramide (Alexa Fluor® is the trademark of ThermoFisher), FITC tyramide, or other comparable fluorescent tyramide conjugates.

Immunofluorescent image of paraffin-embedded human lung carcinoma labeled with  EpCAM Rabbit mAb followed with HRP-labeled goat anti-rabbit IgG (H+L) (Cat#16793) . The signal was developed with iFluor® 488 tyramide or Alexa Fluor™ 488 Tyramide Reagent (Green). Cells were also counterstained with DAPI (Blue).

Protocol

COA

Catalog	Size	Price	Quantity
45100	200 slides	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	188.157 µL	940.787 µL	1.882 mL	9.408 mL	18.816 mL
5 mM	37.631 µL	188.157 µL	376.315 µL	1.882 mL	3.763 mL
10 mM	18.816 µL	94.079 µL	188.157 µL	940.787 µL	1.882 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	531.47 g/mol	=	mL	x	mM	=	mmol

Citations

View all 32 citations: Citation Explorer

Targeting MGLL: Terazosin Regulates Glycerolipid Metabolism to Mitigate Endothelial Cell Senescence

Authors:

Huang, Jie and Yan, Jinhua and Wan, Zixin and Ji, Tianyi and Li, Han and Liang, Wukaiyang and Huang, Yi and Yang, Zhen and Xiao, Yue and Nie, Hao and others,

Journal:

Journal of Lipid Research (2025): 100904

Fatty Acid Transporter CD36 Promotes Ox-LDL-Induced Senescence of Vascular Endothelial Cells in Preeclampsia

Authors:

Xiao, Yanxuan and Tao, Maliang and Yu, Yiqi and Bai, Ruiyan and Zhuang, Yunting and Huang, Qiuyu and He, Jiexing and Lin, Zeshan and Gao, Mingze and Li, Jiaqi and others,

Journal:

Journal of Inflammation Research (2025): 12801--12816

Immunostimulatory DNA Tetrahedron-Based Nanovaccine Combined With Immune Checkpoint PD-1 Blockade for Boosting Systemic Immune Responses Against Oral Squamous Cell Carcinoma

Authors:

Yang, Xueting and Wang, Yun and Yang, Yan and Zhang, Sicheng and Wang, Dianri and Luo, Yi and Shui, Chunyan and Cai, Yongcong and Yang, Ruoyi and Dong, Shuang and others,

Journal:

Aggregate (2025): e70061

The TFIID subunit Taf13 is dispensable for TBP promoter recruitment and RNA polymerase II transcription

Authors:

Martianov, Igor and Le Gras, Stephanie and Davidson, Guillaume and Davidson, Irwin

Journal:

iScience (2025)

In situ protein corona--camouflaged supramolecular assemblies remodel thrombotic microenvironment for improved arterial homeostasis

Authors:

Chen, Dan and Chen, Yifan and Liu, Jianwen and Liu, Xinyue and Liu, Peiwen and Zhan, Jiabing and Chen, Zhiting and Gan, Yong and Huang, Mingdong and Chen, Zhaoyang

Journal:

Science Advances (2025): eadu6676

References

View all 74 references: Citation Explorer

Tyramide Signal Amplification for Immunofluorescent Enhancement

Authors:

Faget L, Hnasko TS.

Journal:

Methods Mol Biol (2015): 161

Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification

Authors:

Trang NT, Hirai T, Ngan PH, Lan NT, Fuke N, Toyama K, Yamamoto T, Yamaguchi R.

Journal:

J Vet Diagn Invest (2015): 326

Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification

Authors:

Aydin M, Herzig GP, Jeong KC, Dunigan S, Shah P, Ahn S.

Journal:

J Food Prot (2014): 100

Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis

Authors:

Stack EC, Wang C, Roman KA, Hoyt CC.

Journal:

Methods (2014): 46

KSHV cell attachment sites revealed by ultra sensitive tyramide signal amplification (TSA) localize to membrane microdomains that are up-regulated on mitotic cells

Authors:

Garrigues HJ, Rubinchikova YE, Rose TM.

Journal:

Virology (2014): 75

Physical properties

Molecular weight	531.47
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 6, 2025