Name: iFluor® 647 Tyramide
Brand: AAT Bioquest
SKU: 45110
Price: 355 USD
Availability: InStock

iFluor® 647 Tyramide

iFluor® 647 Tyramide is a far-red fluorescent signal amplification reagent designed for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Detection Principle: Uses HRP-catalyzed covalent deposition of fluorescent tyramide.
Spectral Properties: Excitation/emission maxima of 655/670 nm.
Filter Compatibility: Compatible with Cy5 fluorescence microscope filter sets.
Applications: Suitable for immunocytochemistry, immunofluorescence, and immunohistochemistry.

Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using iFluor® 647 tyramide (Cat No. 45110) and detected with a Cy5 filter set. Nuclei (blue) were counterstained with DAPI (Cat No. 17507).

Protocol

SDS

Catalog	Size	Price	Quantity
45110	200 Slides	Price

Overview

For many immunohistochemical (IHC) applications, traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit their sensitivity and utility. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label results in the ability to detect low-abundance targets with ultrasensitive precision and reduces the amount of antibodies and hybridization probes needed. In IHC applications, this method can also enhance sensitivity in cases where the primary antibody dilution needs to be increased to reduce nonspecific background signals or overcome weak immunolabeling due to suboptimal fixation procedures or low levels of target expression. The iFluor® 647 tyramide contains the bright iFluor® 647 that can be readily detected with the standard Cy5 filter set. It is an excellent replacement for Alexa Fluor® 647 tyramide (Alexa Fluor® is the trademark of ThermoFisher), Cy5 tyramide, or other comparable fluorescent tyramide conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 Tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	91.558 µL	457.792 µL	915.583 µL	4.578 mL	9.156 mL
5 mM	18.312 µL	91.558 µL	183.117 µL	915.583 µL	1.831 mL
10 mM	9.156 µL	45.779 µL	91.558 µL	457.792 µL	915.583 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1092.2 g/mol	=	mL	x	mM	=	mmol

Citations

View all 15 citations: Citation Explorer

CD46 and DSG2 synergistically mediate human adenovirus type 7 infection

Authors:

Ye, Lihua and Mo, Chuncong and Yuan, Jinwei and Su, Yalin and Zhou, Chengxing and Yang, Yujie and Li, Xiao and Liu, Wenkuan and Zeng, Rongjie and Zhou, Rong and others

Journal:

Journal of Virology (2026): e00136--26

Migrasome-transported PTGES amplifies the PGE2 cascade in SPP1+ macrophages to drive oral leukoplakia carcinogenesis

Authors:

Jiang, Ming-Jing and Zhou, Hao-Yu and Bai, Yu-Ting and Chen, Xiao-Jie and Zhou, Gang

Journal:

Nature Communications (2026)

Omics insights into MYBL2-promoted cancer stem-like cells driving ferroptosis resistance in hepatocellular carcinoma

Authors:

Chen, Yuting and Li, Xu and Luo, Chaochao and Lin, Guanchuan and Zhou, Xuewen and Wen, Zebin and Su, Huanhou and Meng, Wei and Chen, Caiming and Lu, Jiameng and others

Journal:

Journal of Advanced Research (2026)

The impact of elevated temperature and salinity on microbial communities and food selectivity in heterotrophic nanoflagellates in the Boye River

Authors:

Boden, Lisa and Bludau, Dana and Sieber, Guido and Deep, Aman and Baikova, Daria and David, Gwendoline M and Had{\v{z}}iomerovi{\'c}, Una and Stach, Tom L and Boenigk, Jens

Journal:

ISME Communications (2025): ycaf049

H3K9 lactylation in malignant cells facilitates CD8+ T cell dysfunction and poor immunotherapy response

Authors:

Wang, Ruijie and Li, Chuwen and Cheng, Zhongyi and Li, Mingyu and Shi, Jianbo and Zhang, Zhiyuan and Jin, Shufang and Ma, Hailong

Journal:

Cell Reports (2024)

References

View all 4 references: Citation Explorer

Quantum dot-based FRET for sensitive determination of hydrogen peroxide and glucose using tyramide reaction.

Authors:

Huang, Xiangyi and Wang, Jinjie and Liu, Heng and Lan, Tao and Ren, Jicun

Journal:

Talanta (2013): 79-84

Dual enhancement of triple immunofluorescence using two antibodies from the same species.

Authors:

Nakamura, Ayako and Uchihara, Toshiki

Journal:

Journal of neuroscience methods (2004): 67-70

Rapid detection and enumeration of Naegleria fowleri in surface waters by solid-phase cytometry.

Authors:

Pougnard, Claire and Catala, Philippe and Drocourt, Jean-Louis and Legastelois, Stephane and Pernin, Pierre and Pringuez, Emmanuelle and Lebaron, Philippe

Journal:

Applied and environmental microbiology (2002): 3102-7

Oligonucleotides as hybridization probes to localize phytoplasmas in host plants and insect vectors.

Authors:

Webb, D R and Bonfiglioli, R G and Carraro, L and Osler, R and Symons, R H

Journal:

Phytopathology (1999): 894-901

Physical properties

Molecular weight	1092.20
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 6, 2026