iFluor® 633 tyramide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1271.67 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.062 |
Correction Factor (280 nm) | 0.044 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 640 |
Emission (nm) | 654 |
Quantum yield | 0.291 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Molecular weight 1271.67 | Correction Factor (260 nm) 0.062 | Correction Factor (280 nm) 0.044 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 640 | Emission (nm) 654 | Quantum yield 0.291 |
For many immunohistochemical (IHC) applications, traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit their sensitivity and utility. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label results in the ability to detect low-abundance targets with ultrasensitive precision and reduces the amount of antibodies and hybridization probes needed. In IHC applications, this method can also enhance sensitivity in cases where the primary antibody dilution needs to be increased to reduce nonspecific background signals or overcome weak immunolabeling due to suboptimal fixation procedures or low levels of target expression. The iFluor® 633 tyramide contains the bright iFluor® 633 that can be readily detected with the standard Cy5 filter set. iFluor® dyes have higher fluorescence intensity, increased photostability, and enhanced water solubility, resulting in fluorescence signals with significantly higher precision and sensitivity. iFluor® 633 tyramide is an ideal probe for applications where the common tyramides may face interference due to the inherent fluorescence of tissues or other samples.
Platform
Fluorescence microscope
Excitation | Cy5 filter set |
Emission | Cy5 filter set |
Recommended plate | Black wall/clear bottom |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 633 tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 78.637 µL | 393.184 µL | 786.368 µL | 3.932 mL | 7.864 mL |
5 mM | 15.727 µL | 78.637 µL | 157.274 µL | 786.368 µL | 1.573 mL |
10 mM | 7.864 µL | 39.318 µL | 78.637 µL | 393.184 µL | 786.368 µL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.062 |
Correction Factor (280 nm) | 0.044 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 640 |
Emission (nm) | 654 |
Quantum yield | 0.291 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 488 tyramide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 633 maleimide | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
iFluor® 555 Tyramide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 Tyramide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 350 Tyramide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 546 Tyramide | 541 | 557 | 1000001 | 0.671 | 0.25 | 0.15 |
iFluor® 568 Tyramide | 568 | 587 | 1000001 | 0.571 | 0.34 | 0.15 |
iFluor® 594 Tyramide | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 633 Styramide *Superior Replacement for Opal 650* | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
Show More (2) |
References
View all 50 references: Citation Explorer
Tyramide Signal-Amplified Immunofluorescence of MYCN and MYC in Human Tissue Specimens and Cell Line Cultures.
Authors: Schafer, Johanna M and Pietenpol, Jennifer A
Journal: Bio-protocol (2020): e3677
Authors: Schafer, Johanna M and Pietenpol, Jennifer A
Journal: Bio-protocol (2020): e3677
Cascade signal amplification for sensitive detection of exosomes by integrating tyramide and surface-initiated enzymatic polymerization.
Authors: Huang, Zhipeng and Lin, Qiuyuan and Yang, Bin and Ye, Xin and Chen, Hui and Weng, Wenhao and Kong, Jilie
Journal: Chemical communications (Cambridge, England) (2020): 12793-12796
Authors: Huang, Zhipeng and Lin, Qiuyuan and Yang, Bin and Ye, Xin and Chen, Hui and Weng, Wenhao and Kong, Jilie
Journal: Chemical communications (Cambridge, England) (2020): 12793-12796
Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97
Characterizing the Tumor Immune Microenvironment with Tyramide-Based Multiplex Immunofluorescence.
Authors: Mori, Hidetoshi and Bolen, Jennifer and Schuetter, Louis and Massion, Pierre and Hoyt, Clifford C and VandenBerg, Scott and Esserman, Laura and Borowsky, Alexander D and Campbell, Michael J
Journal: Journal of mammary gland biology and neoplasia (2020): 417-432
Authors: Mori, Hidetoshi and Bolen, Jennifer and Schuetter, Louis and Massion, Pierre and Hoyt, Clifford C and VandenBerg, Scott and Esserman, Laura and Borowsky, Alexander D and Campbell, Michael J
Journal: Journal of mammary gland biology and neoplasia (2020): 417-432
Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies.
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)
Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish.
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409
Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins.
Authors: Dopie, Joseph and Sweredoski, Michael J and Moradian, Annie and Belmont, Andrew S
Journal: The Journal of cell biology (2020)
Authors: Dopie, Joseph and Sweredoski, Michael J and Moradian, Annie and Belmont, Andrew S
Journal: The Journal of cell biology (2020)
Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification.
Authors: Zhang, Shouping and Hu, Bin and Xia, Xiaojing and Xu, Yanzhao and Hang, Bolin and Jiang, Jinqing and Hu, Jianhe
Journal: Molecules (Basel, Switzerland) (2019)
Authors: Zhang, Shouping and Hu, Bin and Xia, Xiaojing and Xu, Yanzhao and Hang, Bolin and Jiang, Jinqing and Hu, Jianhe
Journal: Molecules (Basel, Switzerland) (2019)
An ultrasensitive electrochemical immunosensor for procalcitonin detection based on the gold nanoparticles-enhanced tyramide signal amplification strategy.
Authors: Liu, Pei and Li, Chao and Zhang, Ruixuan and Tang, Qing and Wei, Jia and Lu, Yan and Shen, Pingping
Journal: Biosensors & bioelectronics (2019): 543-550
Authors: Liu, Pei and Li, Chao and Zhang, Ruixuan and Tang, Qing and Wei, Jia and Lu, Yan and Shen, Pingping
Journal: Biosensors & bioelectronics (2019): 543-550
A amperometric immunosensor for sensitive detection of circulating tumor cells using a tyramide signal amplification-based signal enhancement system.
Authors: Zhou, Xiaoyan and Li, Yujian and Wu, Haiping and Huang, Wei and Ju, Huangxian and Ding, Shijia
Journal: Biosensors & bioelectronics (2019): 88-94
Authors: Zhou, Xiaoyan and Li, Yujian and Wu, Haiping and Huang, Wei and Ju, Huangxian and Ding, Shijia
Journal: Biosensors & bioelectronics (2019): 88-94