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iFluor® 430 Tyramide
Superior Replacement for Opal 480
iFluor® 430 tyramide is optimized to a superior replacement for Opal 480 or other spectrally similar fluorescent tyramide conjugates or TSA reagents. Tyramide reagetns can be used to detect extremely low-abundance targets in cells and tissues with significantly improved fluorescence signal than the direct fluorescence labeling reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled tyramide conjugates can generate fluorescence signal with significantly higher precision and sensitivity.
<strong>Superior sensitivity with iFluor® 430 tyramide.</strong> HeLa cells were incubated with primary anti-tubulin antibodies followed by detection with HRP-Goat anti-Mouse&nbsp;IgG and&nbsp;iFluor® 430 tyramide&trade; (Left) or Alexa Fluor&reg; 430 tyramide (Right). Fluorescence images were taken on a Keyence BZ-X710 fluorescence microscope equipped with a violet filter set.
<strong>Superior sensitivity with iFluor® 430 tyramide.</strong> HeLa cells were incubated with primary anti-tubulin antibodies followed by detection with HRP-Goat anti-Mouse&nbsp;IgG and&nbsp;iFluor® 430 tyramide&trade; (Left) or Alexa Fluor&reg; 430 tyramide (Right). Fluorescence images were taken on a Keyence BZ-X710 fluorescence microscope equipped with a violet filter set.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
45096200 Slides
Price
 
Physical properties
Molecular weight710.89
SolventDMSO
Spectral properties
Correction factor (260 nm)0.68
Correction factor (280 nm)0.3
Extinction coefficient (cm -1 M -1)
40000
1
Excitation (nm)433
Emission (nm)498
Quantum yield
0.78
1
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationViolet filter set
EmissionViolet filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2025