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AAT Bioquest

How is acetylcholinesterase activity measured?

Posted March 27, 2024


Answer

Acetylcholinesterase is a cholinergic enzyme that catalyzes the breakdown of the neurotransmitter into acetylcholine into acetic acid and choline. 

Acetylcholinesterase activity can be measured via two methods. The first is by quantifying how much choline is produced after a brief interval. The second is by observing the intensification in light emission generated by the buildup of choline. Acetylcholinesterase measurement assay is sensitive and rapid. 

Additional resources

Determination of acetylcholinesterase activity by a new chemiluminescence assay with the natural substrate

Enzymes

Amplite® Colorimetric Acetylcholinesterase Assay Kit