How much time does it take to make a plasmid?

Making a plasmid involves inserting a PCR product into a vector, which takes about five hours of hands-on work for a single plasmid. However, if one is combining multiple PCR products or encounter errors, the time can increase significantly. The majority of researchers have reported it taking over 22 hours, taking nearly four days to complete the process. Thus, making plasmids is a time-intensive task that requires careful attention.