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What are examples of chemiluminescent substrates?
Posted June 24, 2024
Antibodies and Proteomics Chemical ReagentsEnzymesHorseradish Peroxidase (HRP) and Poly-HRPLuciferasesPhysiological Probeschemiluminescence
Answer

Examples of chemiluminescent substrates used with horseradish peroxidase (HRP) include luminol and acridan-based reagents. Alkaline phosphatase can utilize chemiluminescent substrates such as 4-methylumbelliferyl phosphate (4-MUP) or adamantyl 1,2-dioxetane aryl phosphate (AMPPD). Additionally, luciferase enzymes trigger light emission by oxidizing luciferins. Common chemiluminescent substrates for luciferase include luciferin and coelenterazine.

Additional resources
Chapter Nineteen - Western Blotting using Chemiluminescent SubstratesLuminol [3-Aminophthalhydrazide] *CAS 521-31-3*

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