What is acceptor photobleaching? | AAT Bioquest

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What is acceptor photobleaching?

Posted May 15, 2024

Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes

Answer

Acceptor photobleaching is a technique used in FRET experiments to confirm the occurrence of energy transfer between a donor and acceptor fluorophore pair. The rise in donor fluorescence following complete acceptor photobleaching serves as an indicator of the extent of FRET.

Additional resources

Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching Förster Resonance Energy Transfer (FRET) Screen Quest™ TR-FRET No Wash cAMP Assay Kit FMOC-Asp(5-FAM)-OH

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