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What does spectral overlap mean?
Posted May 15, 2024
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes
Answer
Spectral overlap refers to the process where the emission spectrum of one fluorophore (the molecule emitting light) overlaps with the excitation spectrum of another fluorophore (the molecule absorbing light). 
Additional resources
Fluorescence Resonance Energy Transfer (FRET) MicroscopyFörster Resonance Energy Transfer (FRET) Screen Quest™ TR-FRET No Wash cAMP Assay Kit FMOC-Asp(5-FAM)-OH

What methods are used to measure FRET for cell biology studies?What factors affect the degree of spectral overlap?What techniques can be used for FRET quantification?What organic dyes are commonly used as fluorescent probes in FRET?What should I consider when selecting donor/acceptor FRET pairs?